Multiple fluorescence in situ hybridization.

P M Nederlof; S van der Flier; J Wiegant; A K Raap; H J Tanke; J S Ploem; M van der Ploeg

doi:10.1002/cyto.990110115

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Multiple fluorescence in situ hybridization.

P M Nederlof·S van der Flier·J Wiegant·A K Raap·H J Tanke·J S Ploem·M van der Ploeg

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Abstract

A method for multiple fluorescence in situ hybridization is described allowing the simultaneous detection of more than three target sequences with only three fluorescent dyes (FITC, TRITC, AMCA), respectively emitting in the green, red, and blue. This procedure is based on the labeling of (DNA) probes with more than one hapten and visualisation in multiple colors. The possibility to detect multiple targets simultaneously is important for prenatal diagnosis and the detection of numerical and/or structural chromosome aberrations in tumor diagnosis. It may form the basis for an in situ hybridization based chromosome banding technique.

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2-Acetylaminofluorene Biotin Chromosomes Chromosomes, Human, Pair 1 Cytogenetics DNA Probes Deoxyuracil Nucleotides Fluorescent Dyes Humans Male Microscopy, Fluorescence Nucleic Acid Hybridization Signal Processing, Computer-Assisted Journal Article

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P M Nederlof, S van der Flier, J Wiegant, A K Raap, H J Tanke, J S Ploem, M van der Ploeg. 1990. Multiple fluorescence in situ hybridization.. https://doi.org/10.1002/cyto.990110115

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