A note on sequence-dependence of DNA structure.

A Galat

doi:10.1007/bf00258382

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A note on sequence-dependence of DNA structure.

A Galat

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Abstract

A circular dichroism study was conducted on the solution structure of several different oligonucleotides, whose X-ray structures have been solved. It is suggested that in aqueous solution the oligonucleotides can form structures that maintain geometrical elements which are typical of B-DNA, A-DNA, and their intermediate forms. It is shown that 5'GGATGGGAG:5'CTCCCATCC, which forms an A-DNA helix in the crystal state (McCall et al. 1986), in aqueous solution maintains an A-DNA like structure at temperatures below 10 degrees C. At temperatures between 10 degrees C and 25 degrees C it shows a tendency to form an intermediate structure between A-DNA and B-DNA. Also, it is shown that TFE does not cause a transition from B-DNA to A-DNA helix in short DNA fragments, but instead disrupts the helix.

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Base Sequence Calorimetry Circular Dichroism DNA Models, Molecular Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Denaturation Oligodeoxyribonucleotides Software Journal Article Research Support, Non-U.S. Gov't

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A Galat. 1990. A note on sequence-dependence of DNA structure.. https://doi.org/10.1007/bf00258382

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