Paralog chromatography.

L M Kauvar; P Y Cheung; R H Gomer; A A Fleischer

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PubMed · 2317377

Paralog chromatography.

L M Kauvar·P Y Cheung·R H Gomer·A A Fleischer

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Abstract

As a mixed mode ligand, a small peptide can mimic an antibody's paratope (antigen recognition site). This report describes the construction of a representative set of paratope analogs, or "paralogs," which can be conjugated to a chromatographic sorbent to combine desirable characteristics of traditional high-performance liquid chromatography columns with the specificity of a moderate affinity antibody. The broad utility of this novel set of protein separatory reagents is illustrated on the complex mixture of proteins in a yeast lysate.

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Antibodies Cell Fractionation Chromatography Electrophoresis, Polyacrylamide Gel Fungal Proteins Peptides Protein Conformation Yeasts Journal Article

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L M Kauvar, P Y Cheung, R H Gomer, A A Fleischer. 1990. Paralog chromatography.. https://pubmed.ncbi.nlm.nih.gov/2317377/

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