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Epidermal growth factor and phorbol ester regulate prolactin gene expression via distinct pathways.

A E Jackson·S K Bandyopadhyay·C Bancroft
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Abstract

Previous studies, involving phosphorylation of cytoplasmic proteins and localization of DNA regulatory elements, have suggested that epidermal growth factor (EGF) and 12-O-tetradecanoylphorbol-13-acetate (TPA) have similar actions on prolactin (PRL) gene expression by pituitary (GH) cells. However, little is presently known about whether the actions of these two factors involve common gene-distal intermediates. In the present study, we have employed two approaches to examine this question. Chronic exposure of GH3 cells to TPA, which strongly down-regulates protein kinase C activity, completely inhibited acute TPA stimulation of transient expression of a transfected PRL promoter construct ((-187)PRL-CAT), but did not inhibit EGF stimulation of either accumulation of endogenous PRL mRNA or of expression of (-187)PRL-CAT. Furthermore, the acute stimulatory effects of EGF and TPA on expression of (-187)PRL-CAT were additive. Each of these observations implies that EGF and TPA have gene-distal actions on PRL gene expression that are at least partially non-overlapping.

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AnimalsCell LineEpidermal Growth FactorGene Expression RegulationPituitary GlandProlactinPromoter Regions, GeneticProtein Kinase CRatsTetradecanoylphorbol AcetateTranscription, GeneticTransfectionJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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A E Jackson, S K Bandyopadhyay, C Bancroft. 1990-02-12. Epidermal growth factor and phorbol ester regulate prolactin gene expression via distinct pathways.. https://doi.org/10.1016/0303-7207(90)90094-o

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