Quantitative footprinting analysis. Binding to a single site.

R Rehfuss; J Goodisman; J C Dabrowiak

doi:10.1021/bi00455a027

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Quantitative footprinting analysis. Binding to a single site.

R Rehfuss·J Goodisman·J C Dabrowiak

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Abstract

The theory for measuring ligand binding constants from footprinting autoradiographic data associated with a single binding site is derived. If the ligand and DNA cleavage agent compete for a common site, the spot intensities are not proportional to the amount of DNA not blocked by ligand. The analysis of a single site is experimentally illustrated by using results for the anticancer drug actinomycin D interacting with the duplex d(TAGCGCTA)2 as probed with the hydrolytic enzyme DNase I.

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Autoradiography Base Sequence Binding Sites DNA Dactinomycin Deoxyribonuclease I Kinetics Ligands Molecular Sequence Data Nucleotide Mapping Oligonucleotide Probes Journal Article Research Support, Non-U.S. Gov't

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R Rehfuss, J Goodisman, J C Dabrowiak. 1990-01-23. Quantitative footprinting analysis. Binding to a single site.. https://doi.org/10.1021/bi00455a027

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