A rapid method for determining the relationship between cDNA clones.

J A Walker; J S Cairns

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PubMed · 2340177

A rapid method for determining the relationship between cDNA clones.

J A Walker·J S Cairns

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Abstract

We describe a technique for rapidly screening the inserts of plasmids for homology to each other by using DNA fragments isolated in agarose gels to probe Southern blots of DNA prepared by the "miniprep" alkaline lysis method. The procedure includes a technique for labeling DNA fragments in agarose gel slices without further purification. The protocol results in a significant savings in time and expense and a considerable increase in fragment yield over methods involving fragment purification from polyacrylamide or agarose gels.

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Base Sequence Blotting, Southern DNA DNA Probes Methods Nucleic Acid Hybridization Plasmids Sequence Homology, Nucleic Acid Comparative Study Journal Article

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J A Walker, J S Cairns. 1990. A rapid method for determining the relationship between cDNA clones.. https://pubmed.ncbi.nlm.nih.gov/2340177/

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