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PubMed · 2346781

Gova/b alloantigen system on human platelets.

J G Kelton·J W Smith·P Horsewood·J R Humbert·C P Hayward·T E Warkentin
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Abstract

In this report we describe a platelet alloantigen system that is carried on a novel platelet protein of 175 Kd. Antisera against the two alleles (Gova/Govb) were found in two patients who had received large numbers of platelet transfusions. The anti-Gov alloantibodies could not be detected using a whole platelet solid phase enzyme immunoassay, or by a platelet glycoprotein capture enzyme immunoassay using monoclonal antibodies against glycoproteins Ib/IX, Ia/IIa, and IIb/IIIa. Using radioimmunoprecipitation techniques, a protein was precipitated that migrated at 175 Kd (reduced). Under nonreduced conditions, a 150-Kd protein was detected with a minor component at 175Kd. The detection of the alloantigens was not activation-dependent. Using immunodepletion studies, we demonstrated that each alloantiserum recognized an epitope on a discrete population of the 175-Kd platelet protein. Family studies demonstrated that the alloantigens designated as Gova and Govb were inherited in an autosomal codominant fashion. The phenotypic frequencies were Gova/Gova, 26%; Gova/Govb, 55%; Govb/Govb, 19%; giving gene frequencies of 0.532 and 0.468 for Gova and Govb, respectively (n = 33).

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AllelesBlood PlateletsFemaleHumansIntegrin alpha2IsoantigensMembrane GlycoproteinsPedigreePlatelet Membrane GlycoproteinsPrecipitin TestsJournal ArticleResearch Support, Non-U.S. Gov't

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J G Kelton, J W Smith, P Horsewood, J R Humbert, C P Hayward, T E Warkentin. 1990-06-01. Gova/b alloantigen system on human platelets.. https://pubmed.ncbi.nlm.nih.gov/2346781/

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