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Dynamics in synthetic oligonucleotides. A solid-state deuterium NMR study.

T M Alam·G Drobny
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Abstract

Solid-state 2H NMR spectroscopy was used to investigate dynamics in the [methyl-2H]thymidine-labeled oligonucleotide [d(CGCGAAT*T*CGCG)]2. Quadrupole echo line shapes, spin-lattice relaxation, and quadrupolar echo decay rates were investigated as a function of hydration W (moles of water/moles of nucleotide) between 0 and approximately 30. The amplitude of the base motion, modeled as a fast four-site libration, or diffusion in a cone, increased slightly with higher levels of hydration. A slower component of motion about the helix axis appears at W approximately 10 and increases in rate and amplitude, leading to the intermediate rate line shape observed at W approximately 21.

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DeuteriumIsotope LabelingMagnetic Resonance SpectroscopyMathematicsNucleic Acid ConformationOligodeoxyribonucleotidesJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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T M Alam, G Drobny. 1990-04-10. Dynamics in synthetic oligonucleotides. A solid-state deuterium NMR study.. https://doi.org/10.1021/bi00466a001

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