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HELP (high efficiency liquid phase) new oligonucleotide synthesis on soluble polymeric support.

G M Bonora·C L Scremin·F P Colonna·A Garbesi
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Abstract

A simple, rapid and high-yielding method for the synthesis of oligonucleotides by the phosphotriesters approach is described. The use of polyethylene glycol (PEG) as soluble polymeric support preserves some convenient features of the solid-phase synthesis with new interesting advantages. Short oligonucleotides in hundred milligrams scale can be obtained from few grams of functionalized PEG.

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Chromatography, High Pressure LiquidChromatography, Ion ExchangeMagnetic Resonance SpectroscopyOligonucleotidesPolyethylene GlycolsSolubilityJournal ArticleResearch Support, Non-U.S. Gov't

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G M Bonora, C L Scremin, F P Colonna, A Garbesi. 1990-06-11. HELP (high efficiency liquid phase) new oligonucleotide synthesis on soluble polymeric support.. https://doi.org/10.1093/nar%2F18.11.3155

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