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PubMed · 236296

Galactose-6-phosphate dehydrogenase. Purification and partial characterization.

M Ray·A Bhaduri
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Abstract

A new enzyme, galactose-6-phosphate dehydrogenase has been purified about 50-fold from goat liver. The enzyme can be distinguished from the nonspecific hexose-6-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase by its high substrate specificity and absolute pyridine nucleotide requirement. In contrast to the hexose-6-phosphate dehydrogenase, this enzyme is located exclusively in the cytoplasmic fraction of the cell. The enzyme is a metalloprotein and is highly sensitive to mercurials. The product of the reaction is possibly a ketoaldose, phosphorylated at the primary alcoholic group.

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Alcohol OxidoreductasesAnimalsCarbohydrate DehydrogenasesCations, DivalentCations, MonovalentChloromercuribenzoatesChromatography, DEAE-CelluloseCytoplasmEdetic AcidGalactoseGoatsHexosephosphatesHydrogen-Ion ConcentrationIodoacetatesIronKineticsLiverMercaptoethanolNADNADPProtaminesJournal Article

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M Ray, A Bhaduri. 1975-05-25. Galactose-6-phosphate dehydrogenase. Purification and partial characterization.. https://pubmed.ncbi.nlm.nih.gov/236296/

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