Cell spreading on laminin substrate involves Con A-binding proteins.

R Moutsita; J Botti; M A Doyennette-Moyne; M Aubery; P Codogno

doi:10.1051/rnd:19900312

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Cell spreading on laminin substrate involves Con A-binding proteins.

R Moutsita·J Botti·M A Doyennette-Moyne·M Aubery·P Codogno

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Abstract

Concanavalin A (Con A), a tetravalent lectin, was shown to impair 8 chick embryo fibroblast (8 d CEF) spreading on a laminin (LM) substrate but not on a fibronectin substrate (FN), suggesting that cell surface Con A binding proteins could be involved in 8 d CEF spreading on a LM substrate. The interaction of Con A-binding proteins with Con A is dependent upon the carbohydrate moieties of the isolated glycoproteins; since they interact strongly with Con A-Sepharose and are eluted with 0.3 Mol/l alpha-methylmannopyranoside, the isolated Con A binding-proteins inhibit 8 d CEF adhesion to a Con A substrate to the same extent as alpha-methylmannopyranoside. Furthermore, the isolated Con A binding proteins specifically inhibit in a dose-dependent manner 8 d CEF spreading on LM but not on FN.

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Animals Binding Sites Cell Adhesion Chick Embryo Concanavalin A Fibroblasts Glycoproteins Laminin Methylmannosides Receptors, Concanavalin A Journal Article Research Support, Non-U.S. Gov't

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R Moutsita, J Botti, M A Doyennette-Moyne, M Aubery, P Codogno. 1990. Cell spreading on laminin substrate involves Con A-binding proteins.. https://doi.org/10.1051/rnd%3A19900312

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