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Isoelectrofocusing in acetate membrane: the method and application.

S Harada
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Abstract

Acetate membrane as a new medium for isoelectrofocusing was used in this study. The acetate membrane was specially produced for isoelectrofocusing, and contains a surface active agent. The ampholine solution system and isoelectric condition for this purpose are described. Polymorphic traits as alpha1-antitrypsin in human serum and 6-phosphogluconate dehydrogenase (6-PGD) in human erythrocyte could be identified by this method. The isoelectric patterns and the isoelectric points of these proteins were demonstrated on the acetate membrane.

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AcetatesErythrocytesGenetic VariationHumansHydrogen-Ion ConcentrationIsoelectric FocusingPhenotypePhosphogluconate Dehydrogenasealpha 1-AntitrypsinJournal Article

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S Harada. 1975-09-16. Isoelectrofocusing in acetate membrane: the method and application.. https://doi.org/10.1016/0009-8981(75)90048-0

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