Multiple labeling of cellular constituents by combining surface reflection interference and fluorescence microscopy.

M Opas; V I Kalnins

doi:10.1159/000163318

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Multiple labeling of cellular constituents by combining surface reflection interference and fluorescence microscopy.

M Opas·V I Kalnins

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Abstract

In this paper the technique for visualizing cytoskeleton in detergent-extracted cultured cells by surface reflection interference (SRI) microscopy after staining with the protein dye Coomassie Brilliant Blue (SRI-CooB technique) is used in conjunction with fluorescently-labeled antibodies or with other fluorescent probes to detect a number of constituents in the same cultured cell. Because SRI-CooB technique preferentially visualizes microfilament bundles along the ventral aspect of cells adhering to a glass substratum, we feel that this simple and rapid technique has great potential in studies of cell-substratum adhesiveness and of adhesion-related cytoskeletal organization.

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Animals Cell Adhesion Cell Line Chickens Cytological Techniques Cytoskeleton Fibronectins Fluorescent Antibody Technique Fluorescent Dyes Guinea Pigs Humans Mice Microscopy, Fluorescence Microscopy, Interference Rosaniline Dyes Staining and Labeling Journal Article Research Support, Non-U.S. Gov't

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M Opas, V I Kalnins. 1985. Multiple labeling of cellular constituents by combining surface reflection interference and fluorescence microscopy.. https://doi.org/10.1159/000163318

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