A rapid method for purification of myelin basic protein.

T Bellini; M Rippa; M Matteuzzi; F Dallocchio

doi:10.1111/j.1471-4159.1986.tb01788.x

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A rapid method for purification of myelin basic protein.

T Bellini·M Rippa·M Matteuzzi·F Dallocchio

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Abstract

A rapid procedure for purification of myelin basic protein has been developed. White matter is delipidated with 2-butanol, and the residue is extracted at pH 7.5 and 8.5. Myelin basic protein is solubilized by extraction in acetate buffer, pH 4.5. The entire procedure requires less than 4 h, and gives homogeneous protein essentially free of protease activity. This procedure can be scaled down to process milligram amounts of white matter; thus it can be very useful for purification of myelin basic protein from very limited amounts of human white matter obtained during surgery.

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Acetates Acetic Acid Animals Brain Chemistry Buffers Butanols Cattle Chromatography Electrophoresis, Polyacrylamide Gel Humans Myelin Basic Protein Peptide Hydrolases Journal Article Research Support, Non-U.S. Gov't

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T Bellini, M Rippa, M Matteuzzi, F Dallocchio. 1986. A rapid method for purification of myelin basic protein.. https://doi.org/10.1111/j.1471-4159.1986.tb01788.x

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