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Staphylococcal alpha-toxin: a structure-function study using a monoclonal antibody.

S Harshman·N Sugg·B Gametchu·R W Harrison
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Abstract

A monoclonal antibody (A-Tox-653.1) selected for its reactivity in a dot immunoblot assay with denatured staphylococcal alpha-toxin has been isolated and its capacity to block the hemolytic and lethal activities of alpha-toxin measured. In addition, 'reactivity with monomer, hexamer, 125I-monoiodinated and CNBr peptides of alpha-toxin was studied. In all cases the reactions of the monoclonal antibody were compared to those obtained with anti-alpha-toxin rabbit hyperimmune serum. We find that while both the monoclonal antibody and the rabbit antiserum react with all forms of alpha-toxin, only the rabbit antiserum blocks hemolytic or lethal activity. Further, the rabbit antiserum reacts with CNBr fragments IV, V ad VII, whereas the monoclonal antibody reacts only with the carboxy terminal CNBr peptide VII. We conclude that, in solution, the carboxy terminal segment of alpha-toxin is relatively free and reaction with the monoclonal antibody neither impedes its binding to the specific receptor on the membrane nor interferes with formation of the hexamer complex.

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AnimalsAntibodies, MonoclonalBacterial ToxinsCollodionCyanogen BromideElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayEpitopesHemolysin ProteinsHemolysisImmunodiffusionMiceMice, Inbred BALB CPeptide FragmentsRabbitsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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S Harshman, N Sugg, B Gametchu, R W Harrison. 1986. Staphylococcal alpha-toxin: a structure-function study using a monoclonal antibody.. https://doi.org/10.1016/0041-0101(86)90200-x

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