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PubMed · 2429661

Inhibition of overall protein and RNA synthesis as a mechanism for the tunicamycin induced decrease in cytochrome P-450 in rat hepatocytes.

Y Singh·V Shirhatti·C T Liu·H W Davies·G Krishna
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Abstract

In rat hepatocytes maintained in culture, cytochrome P-450 and NADPH cytochrome c reductase activities were decreased by tunicamycin in a dose and time dependent fashion. The effect of tunicamycin was mainly due to inhibition of protein synthesis. Tunicamycin decreased L-[35S] methionine incorporation into many proteins, including a 52 kDa cytochrome P-450 isozyme. Tunicamycin also reduced RNA synthesis. These results indicate that tunicamycin decreased cytochrome P-450 levels in hepatocytes by inhibiting protein and RNA synthesis.

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AnimalsCells, CulturedCytochrome P-450 Enzyme SystemKineticsLiverMaleMethionineProtein BiosynthesisRNARatsRats, Inbred StrainsTunicamycinJournal Article

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Y Singh, V Shirhatti, C T Liu, H W Davies, G Krishna. 1986. Inhibition of overall protein and RNA synthesis as a mechanism for the tunicamycin induced decrease in cytochrome P-450 in rat hepatocytes.. https://pubmed.ncbi.nlm.nih.gov/2429661/

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