A novel technique for viable cell determinations.

N P Singh; R E Stephens

doi:10.3109/10520298609109959

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A novel technique for viable cell determinations.

N P Singh·R E Stephens

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Abstract

We have developed a simple method to determine cell viability using two fluorescent dyes, Hoechst 33258 and acridine orange. When these dyes are used in combination, dead cells fluoresce brilliant blue and live cells fluoresce green. This method works over a range of dye concentrations (Hoechst 33258, 0.25-2 micrograms/ml; acridine orange, 1-5.0 micrograms/ml) and the fluorescence spectra of the two dyes are such that only one set of filters is required to visualize the effects of both dyes simultaneously. It is insensitive to a wide range of exogenous serum concentrations and is read with greater uniformity by different observers.

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Acridine Orange Benzimidazoles Bisbenzimidazole Cell Survival Humans Lymphocytes Microscopy, Fluorescence Staining and Labeling Trypan Blue Journal Article Research Support, Non-U.S. Gov't

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N P Singh, R E Stephens. 1986. A novel technique for viable cell determinations.. https://doi.org/10.3109/10520298609109959

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