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Mast cell secretion: differences between immunologic and non-immunologic stimulation.

B R Rosengard·C Mahalik·D E Cochrane
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Abstract

Non-immunologic and immunologic stimulation of mast cells have been compared. Non-immunologic stimulation relys heavily on cellular Ca, is unaffected by neuraminidase treatment, shows a rapid inactivation, and elicits no increase in the incorporation of 3H-methyl groups into the lipid fraction. In contrast, stimulation by immunologic agents relys primarily on extracellular Ca, is inhibited by neuraminidase treatment, shows a comparatively slow rate of inactivation, and causes a significant increase in the incorporation of 3H-methyl groups into the lipid fraction. We found no evidence of cross-inactivation or desensitization between immunologic and non-immunologic agents. However, pretreatment of mast cells with neurotensin desensitized them to subsequent stimulation by compound 48/80. Our results support the hypothesis that immunologic and non-immunologic agents activate exocytotic mast cell secretion via separate mechanisms.

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AnimalsCalciumHistamine ReleaseImmunoglobulin EIn Vitro TechniquesMaleMast CellsMethionineNeuraminidaseNeurotensinOvalbuminRatsStimulation, Chemicalp-Methoxy-N-methylphenethylamineJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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B R Rosengard, C Mahalik, D E Cochrane. 1986. Mast cell secretion: differences between immunologic and non-immunologic stimulation.. https://doi.org/10.1007/bf01966197

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