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PubMed · 2440396

A new experimental model to study oxygen toxicity.

C Michiels·M Raes·J Remacle
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Abstract

An experimental model was developed in order to study the protective effect of antioxidant molecules. Human diploid WI-38 fibroblasts were cultivated under 2 atm of 95% O2. Antioxidants like alpha-tocopherol or superoxide dismutase (SOD) were added respectively in the culture medium or directly inside the cell through a microinjection technique. With both antioxidant molecules a protection was observed. In the control experiment, cells died within 6 or 8 days depending on the confluency and the malonaldehyde content increased sharply. This model represents a new tool in order to test other antioxidant systems towards an oxidative stress.

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AntioxidantsCell LineFibroblastsHumansMicroinjectionsOxygenSuperoxide DismutaseVitamin EJournal ArticleResearch Support, Non-U.S. Gov't

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C Michiels, M Raes, J Remacle. 1986. A new experimental model to study oxygen toxicity.. https://pubmed.ncbi.nlm.nih.gov/2440396/

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