Morphometry and cytometry correlated to quantitative autoradiography.

Abstract

Studies performed in various cell systems and designed to establish correlations between morphometric and functional parameters of individual cells are reviewed. Functional parameters were evaluated by utilizing quantitative 14C-autoradiography and measuring DNA, RNA and protein synthesis. Morphologic parameters were derived from the scanning of Feulgen-stained nuclei and the calculation of features related to shape, optical density and texture. A series of correlations between parameters of these two groups of features was established. Results such as the possibility of allocating cells to the G1, S and G2 phases by textural and densitometric features alone, without making use of the total DNA content and nuclear size, point to the power of this approach. The data, however, are not yet comprehensive enough to allow the interpretation of morphologic parameters in terms of cellular function on a large scale. It is emphasized that the measurement of the RNA synthesis rate will further promote the functional understanding of structural details and may help in making this approach useful for diagnostic purposes.