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PubMed · 2443980

Model substrates for an RNA enzyme.

W H McClain·C Guerrier-Takada·S Altman
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Abstract

M1 RNA, the catalytic RNA subunit of Escherichia coli ribonuclease P, can cleave novel transfer RNA (tRNA) precursors that lack specific domains of the normal tRNA sequence. The smallest tRNA precursor that was cleaved efficiently retained only the domain of the amino acid acceptor stem and the T stem and loop. The importance of the 3' terminal CCA nucleotide residues in the processing of both novel and normal tRNA precursors implies that the same enzymatic function of M1 RNA is involved.

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Base SequenceDNADNA, RecombinantEndoribonucleasesEscherichia coliEscherichia coli ProteinsMolecular Sequence DataMutationNucleic Acid ConformationPlasmidsRNA PrecursorsRNA, BacterialRNA, Transfer, Amino AcylRibonuclease PRibonuclease T1Structure-Activity RelationshipSubstrate SpecificitySuppression, GeneticComparative StudyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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W H McClain, C Guerrier-Takada, S Altman. 1987-10-23. Model substrates for an RNA enzyme.. https://doi.org/10.1126/science.2443980

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