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A quick, reliable staining technique for human sperm morphology.

T F Kruger·S B Ackerman·K F Simmons·R J Swanson·S S Brugo·A A Acosta
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Abstract

The evaluation of sperm morphology is still an important parameter in the diagnosis of the infertile male. Most techniques used for staining human sperm are very time-consuming. A routine stain used for determining differential count of leucocytes (Diff-Quik stain) was evaluated against the standard Papanicolaou stain. Morphology results from 20 duplicate semen smears using both staining methods were determined separately by 2 technicians using a blind protocol. No significant differences were observed when comparing the two staining methods (paired Student's t-test). The advantages of the Diff-Quik stain are: a) complete staining-to-reading time under 7 min, b) commercially prepared reagents, and c) case of staining procedure.

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HumansInfertility, MaleMaleOligospermiaSpermatozoaStaining and LabelingComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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T F Kruger, S B Ackerman, K F Simmons, R J Swanson, S S Brugo, A A Acosta. 1987. A quick, reliable staining technique for human sperm morphology.. https://doi.org/10.3109/01485018708988493

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