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Improved avidin-biotin-peroxidase complex (ABC) staining.

G Cattoretti·E Berti·R Schiró·L D'Amato·C Valeggio·F Rilke
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Abstract

A considerable intensification of the avidin-biotin-peroxidase complex staining system (ABC) was obtained by sequentially overlaying the sections to be immunostained with an avidin-rich and a biotin-rich complex. Each sequential addition contributed to the deposition of horseradish peroxidase on the immunostained site and allowed the subsequent binding of a complementary complex. With this technique a higher dilution of the antisera could be used and minute amounts of antigen masked by the fixative could be demonstrated on paraffin sections.

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AvidinBiotinHistocytochemistryHorseradish PeroxidaseHumansPeroxidasesStaining and LabelingJournal Article

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G Cattoretti, E Berti, R Schiró, L D'Amato, C Valeggio, F Rilke. 1988. Improved avidin-biotin-peroxidase complex (ABC) staining.. https://doi.org/10.1007/bf01746607

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