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Ionic currents in avian granulosa cells.

J L Schwartz·G A Mealing·E K Asem·J F Whitfield·B K Tsang
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Abstract

Transmembrane ionic currents have been recorded in single granulosa cells from the laying hen using the whole-cell patch-clamp technique. Under voltage-clamp conditions, depolarizing voltage steps evoked currents composed of a fast inactivating inward component and a delayed outward component. The former was activated at voltages more positive than -50 mV and was fully inactivated within 500 ms. It was blocked by D600 (methoxyverapamil) and by cobalt, suggesting that it is a calcium current. The latter displayed inward rectification and did not inactivate during long duration pulses. It was blocked by tetraethylammonium indicating that it is a potassium current. This is the first evidence of the existence of potassium and calcium transmembrane currents in granulosa cells.

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AnimalsBariumCalciumCalcium ChannelsCells, CulturedChickensFemaleGallopamilGranulosa CellsIon ChannelsMembrane PotentialsPotassium ChannelsTetraethylammoniumTetraethylammonium CompoundsJournal ArticleResearch Support, Non-U.S. Gov't

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J L Schwartz, G A Mealing, E K Asem, J F Whitfield, B K Tsang. 1988-12-05. Ionic currents in avian granulosa cells.. https://doi.org/10.1016/0014-5793(88)81053-6

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