Precipitation of soluble affinity complexes by a second affinity interaction: a model study.

C Senstad; B Mattiasson

doi:10.1111/j.1470-8744.1989.tb00050.x

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Precipitation of soluble affinity complexes by a second affinity interaction: a model study.

C Senstad·B Mattiasson

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Abstract

Lactate dehydrogenase was purified by affinity precipitation. The enzyme bound to Blue Dextran (the Cibacron blue residues) and was precipitated by addition of concanavalin A. The lectin functions as a crosslinking agent, building up large flocs of dextran that subsequently precipitate, thus co-precipitating the affinity-bound lactate dehydrogenase.

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Animals Chemical Precipitation Coloring Agents Concanavalin A Dextrans Electrophoresis, Polyacrylamide Gel L-Lactate Dehydrogenase Myocardium Proteins Solubility Swine Journal Article Research Support, Non-U.S. Gov't

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C Senstad, B Mattiasson. 1989. Precipitation of soluble affinity complexes by a second affinity interaction: a model study.. https://doi.org/10.1111/j.1470-8744.1989.tb00050.x

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