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A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism.

N Picard-Pasquier·M Ouagued·B Picard·P Goullet·R Krishnamoorthy
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Abstract

A rapid DNA extraction procedure and random primer labelling of Escherichia coli ribosomal RNA with cloned reverse transcriptase have been used to establish a simple and highly sensitive method for studying ribosomal DNA polymorphism in bacteria. Examples of inter- and intraspecies differentiation of bacteria are given and potential applications in bacterial epidemiology and taxonomy are discussed.

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AcinetobacterAutoradiographyBacteriaDNA, BacterialDNA, RibosomalEnterobacteriaceaeEthidiumMethodsPolymorphism, GeneticPolymorphism, Restriction Fragment LengthRNA ProbesRNA-Directed DNA PolymeraseSpecies SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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N Picard-Pasquier, M Ouagued, B Picard, P Goullet, R Krishnamoorthy. 1989. A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism.. https://doi.org/10.1002/elps.1150100306

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