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PubMed · 2469494

High pp60c-src level in human platelet dense bodies.

F Rendu·M Lebret·S Danielian·R Fagard·S Levy-Toledano·S Fischer
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Abstract

Phosphoproteins phosphorylated in vivo were examined in resting and thrombin-activated human blood platelets. Thrombin-stimulation resulted in an overall increase in labeled proteins containing phosphotyrosine. The most prominent was a protein of 60 Kd. By electroblotting, the 60 Kd protein was identified as the pp60c-src, the normal cellular homolog of the transforming protein of Rous sarcoma virus. We have examined the intracellular distribution of the pp60c-src within platelets. Use of immunoprecipitation and electrotransfer to study isolated membranes, alpha-granules, lysosomes, and dense granules (also termed dense bodies) revealed that pp60c-src was highly enriched in dense bodies. In view of the prominent role of these granules in platelet function, We postulate that protein phosphorylation by activated pp60c-src is involved in early steps of platelet activation.

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Blood PlateletsCytoplasmic GranulesHumansMolecular WeightPhosphoproteinsPhosphorylationPhosphotyrosineProto-Oncogene ProteinsProto-Oncogene Proteins pp60(c-src)Subcellular FractionsTyrosineJournal ArticleResearch Support, Non-U.S. Gov't

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F Rendu, M Lebret, S Danielian, R Fagard, S Levy-Toledano, S Fischer. 1989-05-01. High pp60c-src level in human platelet dense bodies.. https://pubmed.ncbi.nlm.nih.gov/2469494/

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