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PubMed · 2476130

Spermine stimulates RNA-dependent reverse transcriptase activity.

H Aoyama
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Abstract

The polyamine spermine can not replace Mg2+ in the reactions catalyzed by avian myeloblastosis virus reverse transcriptase. In the presence of suboptimal Mg2+ concentrations, spermine enhanced the reverse transcriptase activities with viral 70 S RNA and synthetic polyriboadenylic acid as templates, about 4-fold and 2-fold, respectively. A similar effect occurred in the polyriboadenylic acid-directed reaction using Mn2+ as divalent cation. On the other hand, no stimulation by spermine was observed with activated DNA and polydeoxyadenylic acid as template in the reactions catalyzed by reverse transcriptase.

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Avian Leukosis VirusAvian Myeloblastosis VirusCations, DivalentDNAMagnesiumPoly dA-dTRNA, ViralRNA-Directed DNA PolymeraseSpermineTemplates, GeneticJournal ArticleResearch Support, Non-U.S. Gov't

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H Aoyama. 1989. Spermine stimulates RNA-dependent reverse transcriptase activity.. https://pubmed.ncbi.nlm.nih.gov/2476130/

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