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PubMed · 2480180

Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing.

B Bianco·A Diaspro
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Abstract

The properties of an optical microscope are analyzed and analytically evaluated with a simple and effective model in order to understand the true meaning, limitations, and real capabilities of a defocusing technique. Major emphasis is given to the applications related to microscopic objects of biological interest using fluorescence and absorption light microscopy. A procedure for three-dimensional viewing is analyzed and discussed.

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Cytological TechniquesImage Processing, Computer-AssistedMathematicsMicroscopyModels, TheoreticalOptics and PhotonicsJournal Article

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B Bianco, A Diaspro. 1989. Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing.. https://doi.org/10.1007/bf02989683

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