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PubMed · 2481841

Labile protecting groups in tRNA synthesis.

C Chaix·A M Duplaa·D Gasparutto·D Molko·R Téoule
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Abstract

The use of labile protecting groups for the protection of the exocyclic amino function of adenine, guanine and cytosine has two main advantages in RNA synthesis. Final deprotection in concentrated aqueous ammonia takes place in milder conditions which are more compatible with the sensitivity of oligoribonucleotides towards alkali-conditions. The introduction of fragile bases such as certain modified bases encountered in the primary structure of t-RNA is feasible. The chemical synthesis of RNA fragments constituting the primary structure of B. subtilis f-methionine t-RNA is described.

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Bacillus subtilisBase SequenceMolecular Sequence DataPseudouridineRNA, BacterialRNA, Transfer, Amino Acid-SpecificRNA, Transfer, MetUridineJournal Article

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C Chaix, A M Duplaa, D Gasparutto, D Molko, R Téoule. 1989. Labile protecting groups in tRNA synthesis.. https://pubmed.ncbi.nlm.nih.gov/2481841/

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