High-resolution imaging of synaptic structure with a simple confocal microscope.

J W Lichtman; W J Sunderland; R S Wilkinson

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PubMed · 2488274

High-resolution imaging of synaptic structure with a simple confocal microscope.

J W Lichtman·W J Sunderland·R S Wilkinson

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Abstract

We describe a straightforward modification to a conventional fluorescence microscope that permits confocal fluorescence imaging of living and fixed biological material. The modified microscope has been used to examine the morphology of presynaptic terminals at the snake neuromuscular junction. Among our observations is the presence of two discrete compartments within each terminal bouton.

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Animals Fluorescent Dyes Microscopy, Fluorescence Neuromuscular Junction Snakes Synapses Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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J W Lichtman, W J Sunderland, R S Wilkinson. 1989. High-resolution imaging of synaptic structure with a simple confocal microscope.. https://pubmed.ncbi.nlm.nih.gov/2488274/

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