A cell-free model system for the study of antigen processing.

M A Shaw; B M Chain

doi:10.1093/intimm/1.6.619

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A cell-free model system for the study of antigen processing.

M A Shaw·B M Chain

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Abstract

Antigen processing is an essential step in the presentation of most protein antigens to class 2 MHC restricted T cells, but many of the details of processing remain unknown. In this study we show that a whole cell lysate, as well as membrane fractions from an antigen-presenting B cell lymphoma, can process ovalbumin. In this system native ovalbumin incubated with these membranes at acidic pH can be presented to an antigen-specific hybridoma by gluteraldehyde-fixed, antigen-presenting cells. This processing is inhibited by pepstatin, a selective inhibitor of aspartyl proteases. This is the first study of processing by subcellular fractions, and this cell-free system will provide a good model in which to dissect further the molecular requirements of antigen processing.

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Animals Antigen-Presenting Cells Antigens B-Lymphocytes Cell Membrane Cell-Free System Models, Biological Ovalbumin Peptide Hydrolases Subcellular Fractions Tumor Cells, Cultured Journal Article Research Support, Non-U.S. Gov't

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M A Shaw, B M Chain. 1989. A cell-free model system for the study of antigen processing.. https://doi.org/10.1093/intimm%2F1.6.619

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