Rapid method for identifying subclones bridging gaps between contiguous DNA sequences.

Y Y Lu; F D Ni; G F Hong

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PubMed · 2491324

Rapid method for identifying subclones bridging gaps between contiguous DNA sequences.

Y Y Lu·F D Ni·G F Hong

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Abstract

Randomly sequencing of a limited number of unidirectional deletion subclones efficiently generated contiguous DNA sequences with some gaps between them. Gap-bridging subclones were identified by agarose gel electrophoresis. Those located at either end of each individual contiguous sequence were used as standard marker molecules; the subclones between them proved to be correctly identified in most cases. A 4064 bp DNA fragment containing the psr gene was sequenced with the strategy described in this paper.

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Base Sequence Chromosome Deletion Cloning, Molecular DNA Electrophoresis, Agar Gel Journal Article

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Y Y Lu, F D Ni, G F Hong. 1989. Rapid method for identifying subclones bridging gaps between contiguous DNA sequences.. https://pubmed.ncbi.nlm.nih.gov/2491324/

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