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PubMed · 2491328

Protein extraction with reverse micelles.

H Q Shi·L Xia·R Guo·Z Y Shen
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Abstract

Liquid-liquid protein extraction with reverse micelles is a new method for protein separation. We studied the effects of pH, ionic concentration, cation type, and protein molecular mass on performance of an AOT/isooctane reverse micelle system in extraction of lysozyme, trypsin, and pepsin. Extraction was found to be very efficient for low-molecular-mass proteins.

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CationsHydrogen-Ion ConcentrationMethodsMicellesMuramidaseOsmolar ConcentrationPepsin AProtein DenaturationProteinsSaltsTrypsinJournal Article

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H Q Shi, L Xia, R Guo, Z Y Shen. 1989. Protein extraction with reverse micelles.. https://pubmed.ncbi.nlm.nih.gov/2491328/

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