PubMed · 2494714
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F Alam, B V Bapat, A H Soloway, R F Barth, N Mafune, D M Adams. Boronated compounds for neutron capture therapy.. https://pubmed.ncbi.nlm.nih.gov/2494714/
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The interrelationships between activation of phospholipases and neutrophil stimulus-induced Ca2+ responses remain unclear. We report here that immune complexes activate a phosphatidylcholine-specific phospholipase A in a neutrophil only after the cytoplasmic Ca2+ transient has been initiated in the same cell, while chemotactic peptide activation does not proceed via such a phospholipase A-mediated mechanism. Measurements of [Ca2+] changes and of phosphatidylcholine-specific phospholipase A activity were made by flow cytometry, using Indo-1 for Ca2+ indication, and a new fluorescent probe, bis-BODIPY-phosphatidylcholine, localized in the inner leaflet of the plasma membrane, to measure phospholipase A activation. Both 100 nM formyl-methionyl-leucyl-phenylalanine (with or without cytochalasin B) and 60 micrograms/ml insoluble immune complexes elicited cytoplasmic Ca2+ transients, but only insoluble immune complexes stimulated phospholipase A activation in a subpopulation of cells exhibiting an elevation of [Ca2+]in. Phospholipase A activation followed the Ca2+ transient, starting, in each cell, after [Ca2+]in had begun to decrease as Ca2+ redistributed in the activated cell. The products of this phospholipase activation were confirmed by thin layer chromatography. We conclude that neutrophils respond to immune complexes with an elevated cytoplasmic Ca(2+)-requiring phosphatidylcholine-specific phospholipase A activation and to chemotactic peptides by a different mechanism.
Adhesiveness between the resin shell and composite resin was examined. As the resin shell, SR-PE-ISOCETTE, made from thermosetting crown and bridge resin, was used. The shear bond strengths between the resin shell and photocurable composite resin bonded by various methods were measured after 1-day of immersion in water at 37 degrees C. Super-bond C & B treatment to the resin shell effectively improved the adhesiveness, giving a bond strength of about 14 MPa. Clearfil new bond, Clearfil porcelain bond, Unifast and MMA/TBBO treatment gave almost the same bond strengths of about 7-9 MPa. Silane coupling agents were not effective for improving the bond strength. It was revealed that 4-META was necessary for obtaining good adhesion between SR-PE-ISOCETTE and composite resin.
Cell cultures obtained from human uveal tumours have been used as experimental model to study the lethal effect consecutive to the neutron capture reaction on boron incorporated into cells as borophenylalanine. An irradiation with a neutron fluence of 6 x 10(9) n cm-2 reduced the number of viable cells by about 30%.