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Microassay for N-acetyltransferase activity using high-performance liquid chromatography with electrochemical detection.

R J Martin·R G Downer
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Abstract

A rapid, sensitive procedure has been developed for determination of N-acetyltransferase activity against octopamine, dopamine and 5-hydroxytryptamine. The assay, which is performed in a volume of 10 microliters, is based upon the separation and detection of monoamine substrates and their N-acetylated derivatives using high-performance liquid chromatography with electrochemical detection. The method has been used to measure N-acetyltransferase activity against octopamine, dopamine and 5-hydroxytryptamine in the cerebral ganglion of the American cockroach, Periplaneta americana and to study bi-substrate kinetics of the enzyme.

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5-HydroxytryptophanAcetyltransferasesAnimalsChromatography, High Pressure LiquidCockroachesDopamineElectrochemistryMaleOctopamineTemperatureJournal Article

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BibTeXRIS

R J Martin, R G Downer. 1989-02-24. Microassay for N-acetyltransferase activity using high-performance liquid chromatography with electrochemical detection.. https://doi.org/10.1016/s0378-4347(00)83038-8

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