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Cytoplasmic polyhedrosis virus classification by electropherotype; validation by serological analyses and agarose gel electrophoresis.

P P Mertens·N E Crook·R Rubinstein·S Pedley·C C Payne
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Abstract

Serological analyses of several different cytoplasmic polyhedrosis viruses (CPVs), including two type 1 CPVs from Bombyx mori, type 1 CPV from Dendrolimus spectabilis, type 12 CPV from Autographa gamma, type 2 CPV from Inachis io, type 5 CPV from Orgyia pseudotsugata and type 5 CPV from Heliothis armigera, demonstrated a close correlation between the antigenic properties of the polyhedrin or virus particle structural proteins and the genomic dsRNA electropherotypes. The dsRNAs of these viruses were analysed by electrophoresis in 3% and 10% polyacrylamide gels with a discontinuous Tris-HCl/Tris-glycine buffer system or by 1% agarose gel electrophoresis using a continuous Tris-acetate-EDTA buffer system. Electrophoretic analysis in agarose gels was found to be the most suitable for the classification of CPV isolates into electropherotypes, and the results obtained showed a close correlation with the observed antigenic relationships between different virus isolates. However, electrophoretic analysis in 10% polyacrylamide gels was most sensitive for the detection of intra-type variation and the presence of mixed virus isolates.

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AnimalsAntigens, ViralElectrophoresis, Agar GelElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayImmunodiffusionInsect VirusesOcclusion Body Matrix ProteinsRNA, Double-StrandedRNA, ViralViral ProteinsViral Structural ProteinsVirionComparative StudyJournal Article

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P P Mertens, N E Crook, R Rubinstein, S Pedley, C C Payne. 1989. Cytoplasmic polyhedrosis virus classification by electropherotype; validation by serological analyses and agarose gel electrophoresis.. https://doi.org/10.1099/0022-1317-70-1-173

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