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PubMed · 2500830

Visualization of anionic sites using polyethyleneimine-metal complexes.

U Pfüller·K Ichev·W Ovtscharoff·H Franz
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Abstract

17 Polyethyleneimine-metal complexes were synthesized and 3 of them were tested cytochemically for visualization of negative tissue charges. The demonstration of the anionic sites was carried out on rat cerebral cortex and on frog cutaneous pectoral muscle. As controls, neuraminidase digestion, methylation, and omission of osmium-postfixation were used. The osmiophilic properties of polyethyleneimine, polyethyleneimine salts, and polyethyleneimine-metal complexes were discussed.

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AnimalsAnionsCell MembraneCerebral CortexIndicators and ReagentsMetalsMicroscopy, ElectronMusclesPolyethyleneimineRanidaeRatsSarcoplasmic ReticulumSynaptic VesiclesJournal Article

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U Pfüller, K Ichev, W Ovtscharoff, H Franz. 1989. Visualization of anionic sites using polyethyleneimine-metal complexes.. https://doi.org/10.1016/s0065-1281(89)80069-8

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