Logarithmic timebase for stopped-flow data acquisition and analysis.

A R Walmsley; C R Bagshaw

doi:10.1016/0003-2697(89)90315-1

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Logarithmic timebase for stopped-flow data acquisition and analysis.

A R Walmsley·C R Bagshaw

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Abstract

A method for capturing stopped-flow and other rapid reaction records in logarithmic time, using a microcomputer, is described. Apart from the ability to record processes over several decades in time in a single experiment, the method shows distinct advantages in subsequent nonlinear regression analysis of multiexponential processes. The method is illustrated by a study of the binding of NADPH to dihydrofolate reductase and the reaction between formycin triphosphate and heavy meromyosin.

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Animals Formycins Lacticaseibacillus casei Mathematical Computing Microcomputers Mollusca Muscles Myosin Subfragments Ribonucleotides Tetrahydrofolate Dehydrogenase Time Journal Article Research Support, Non-U.S. Gov't

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A R Walmsley, C R Bagshaw. 1989-02-01. Logarithmic timebase for stopped-flow data acquisition and analysis.. https://doi.org/10.1016/0003-2697(89)90315-1

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