Expression of rubella virus cDNA encoding the E1 structural protein.

A de Mazancourt; M Perricaudet

doi:10.1016/0300-9084(89)90162-4

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Expression of rubella virus cDNA encoding the E1 structural protein.

A de Mazancourt·M Perricaudet

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Abstract

cDNAs synthesized from the 40S RNA genome of rubella virus were cloned into the lambda gt10 bacteriophage. A clone (pRV # 1475) which contains a truncated version of the E1 envelope glycoprotein (amino acid residues 17-481) and the 3' non-coding region was constructed from two overlapping cDNAs. pRV # 1475 was inserted into a eukaryotic expression vector under the control of the cytomegalovirus immediate early promoter. After transfection of 293 cells, a protein with intact antigenic domains could be detected.

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Cell Line Cloning, Molecular DNA, Viral Fluorescent Antibody Technique Plasmids RNA, Viral Rubella virus Transfection Viral Envelope Proteins Journal Article Research Support, Non-U.S. Gov't

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A de Mazancourt, M Perricaudet. 1989. Expression of rubella virus cDNA encoding the E1 structural protein.. https://doi.org/10.1016/0300-9084(89)90162-4

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