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Characterization of amplified intracisternal A-particle elements encoding integrase.

K K Lueders·Z Grossman·J W Fewell
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Abstract

Type IIB intracisternal A-particle (IAP) elements have undergone marked amplification and transposition in the genomic DNA of some mouse myelomas. We have made a cDNA library from one such myeloma, MOPC 315, to determine whether some property of the elements themselves has a role in this process. Sequencing of several type IIB cDNAs and one genomic type IIB IAP element has shown that they are nearly identical (greater than 99%) and contain 2 open reading frames (ORFs). ORF2 is capable of encoding the IAP integrase, an enzyme which catalyzes integration of proviral DNA into the genome. An antiserum to a synthetic peptide based on the IAP integrase gene sequence reacted with ORF2 product expressed in bacteria as a fusion protein, and detected a 47 kDa protein, predicted from the size of ORF2, in myeloma cell fractions by Western blotting.

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Amino Acid SequenceAnimalsBase SequenceBlotting, WesternDNA NucleotidyltransferasesDNA, NeoplasmEmbryo, MammalianGene AmplificationGene LibraryGenes, Intracisternal A-ParticleIntegrasesMiceMice, Inbred BALB CMolecular Sequence DataPlasmacytomaProtein BiosynthesisProto-OncogenesRestriction MappingTranscription, GeneticJournal Article

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K K Lueders, Z Grossman, J W Fewell. 1989-11-25. Characterization of amplified intracisternal A-particle elements encoding integrase.. https://doi.org/10.1093/nar%2F17.22.9267

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