Thymic requirement for clonal deletion during T cell development.

A M Fry; L A Jones; A M Kruisbeek; L A Matis

doi:10.1126/science.2511630

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PubMed · 2511630

Thymic requirement for clonal deletion during T cell development.

A M Fry·L A Jones·A M Kruisbeek·L A Matis

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Abstract

During T cell differentiation, self tolerance is established in part by the deletion of self-reactive T cells within the thymus (negative selection). The presence of T cell receptor (TCR)-alpha beta + T cells in older athymic (nu/nu) mice indicates that some T cells can also mature without thymic influence. Therefore, to determine whether the thymus is required for negative selection, TCR V beta expression was compared in athymic nu/nu mice and their congenic normal littermates. T cells expressing V beta 3 proteins are specific for minor lymphocyte stimulatory (Mlsc) determinants and are deleted intrathymically due to self tolerance in Mlsc+ mouse strains. Here it is shown that V beta 3+ T cells are deleted in Mlsc+ BALB/c nu/+ mice, but not in their BALB/c nu/nu littermates. Thus, the thymus is required for clonal deletion during T cell development.

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Animals Antigens, Differentiation, T-Lymphocyte CD4 Antigens CD8 Antigens Flow Cytometry Gene Expression Macromolecular Substances Mice Mice, Inbred BALB C Mice, Inbred Strains Mice, Nude Receptors, Antigen, T-Cell T-Lymphocytes Thymus Gland Journal Article Research Support, Non-U.S. Gov't

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A M Fry, L A Jones, A M Kruisbeek, L A Matis. 1989-11-24. Thymic requirement for clonal deletion during T cell development.. https://doi.org/10.1126/science.2511630

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