PubMed · 251198
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R B Myers. Practical system for validating heat sterilization processes.. https://pubmed.ncbi.nlm.nih.gov/251198/
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The cis and trans forms of thymidine methyl cyclic 3',5'-phosphate (9) and of the corresponding phenylphosphonate derivatives (10) were prepared in two steps from the corresponding cyclic amide. 1H NMR spectroscopy showed the phosphorus-containing six-membered rings of cis-9 (MeO and thymin-1-yl cis) to be in the chair conformation, but trans-9 to be approximately 40% in the twist conformation in pyridine-d6 and 33% in acetone-d6. For trans-9, there was resistance of conversion of the chair form into the twist form with the MeO group pseudo-axial with a delta G degrees(C-->T) value of 3.0 kcal/mol. Similarly, for cis-10 (23-39% twist population in CD3CN, acetone-d6, and CDCl3), a delta G degrees(C-->T) value of 0.5-0.6 kcal/mol for placement of the phosphoryl oxygen pseudo-axial was obtained. Both values are taken to be approximations for delta G degrees(C-->T) for cTMP. For trans-10, the twist conformation was populated to the extent of 6-13% with a delta G degrees(C-->T) value of 1.3 kcal/mol.
Genetically related diploid strains of Saccharomyces cerevisiae that accumulate varied amounts of trehalose during starvation for nitrogen have been constructed. Strains that produced greater than 5% trehalose (dry cell weight) were more tolerant of thermal, or freeze-thaw stresses than strains that produced less than 4% trehalose. Thus trehalose appears to play a role in stress tolerance of yeast. The significance of these results is that, for the first time, a series of related, unmutated strains have been used to test the effect of trehalose on thermotolerance. Previous studies employed either heat shock treatment, or mutated strains to provide trehalose variations, and as such the contribution of the disaccharide to stress tolerance could not necessarily be separated from other factors such as heat shock proteins.