Telomeric sequences derived from laser-microdissected polytene chromosomes.

N Ponelies; E K Bautz; S Monajembashi; J Wolfrum; K O Greulich

doi:10.1007/bf00292388

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Telomeric sequences derived from laser-microdissected polytene chromosomes.

N Ponelies·E K Bautz·S Monajembashi·J Wolfrum·K O Greulich

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Abstract

Telomeric fragments from salivary gland squashes of Drosophila melanogaster Oregon R. were produced by a new microdissection technique, UV laser microbeam dissection. Microdissection, an essential step in microcloning procedures, is usually performed using micromanipulators and microneedles. Recently it has been shown that microdissection can be improved to very high precision if a laser coupled into a microscope is used. A laser microbeam, generated by an excimer pumped dye laser, allows chromosomes to be cut into slices of less than 0.5 micron. Here it is shown, that single copy DNA probes prepared from Drosophila chromosomes by laser microdissection and microcloning relocalize to the chromosomal regions from which they are derived. The combination of laser technique and microcloning provides an advantageous approach for rapid genetic analysis with potential for the study of genetic diseases and genome mapping.

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Animals Blotting, Southern Chromosomes Cloning, Molecular DNA Drosophila melanogaster Fluorescent Antibody Technique Humans Lasers Microscopy, Electron, Scanning Repetitive Sequences, Nucleic Acid Journal Article Research Support, Non-U.S. Gov't

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N Ponelies, E K Bautz, S Monajembashi, J Wolfrum, K O Greulich. 1989. Telomeric sequences derived from laser-microdissected polytene chromosomes.. https://doi.org/10.1007/bf00292388

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