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Labelling oligonucleotides to high specific activity (I).

A G Craig·D Nizetic·H Lehrach
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Abstract

The normal procedure for labelling oligonucleotides radioactively is the use of polynucleotide kinase and gamma 32P-ATP. However, this has the disadvantage of only introducing one labelled base per molecule of the oligonucleotide. In this paper we describe an approach based on primer/template combinations using conventional fill-in conditions followed by the release of the labelled sequence by digestion with uracil-DNA glycosylase.

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Bacteriophage lambdaBase SequenceDNA, ViralMolecular Probe TechniquesNucleic Acid HybridizationOligonucleotide ProbesTemplates, GeneticJournal Article

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A G Craig, D Nizetic, H Lehrach. 1989-06-26. Labelling oligonucleotides to high specific activity (I).. https://doi.org/10.1093/nar%2F17.12.4605

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