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PubMed · 2531734

Assay method for single-chain urokinase-type plasminogen activator.

O Matsuo·S Ueshima·K Okada·H Fukao·M Ohgaki·R Watanabe·H Arimura
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Abstract

A new assay method for measuring single-chain urokinase-type plasminogen activator (scu-PA) activity by quantitative calorimetric assay method is described. The serine enzyme u-PA activates plasminogen to plasmin, and scu-PA is a precursor protein obtained from a tissue culture of human kidney cells. The scu-PA solution was activated by plasmin to two-chain u-PA, which releases p-nitroaniline from pyro-Glu-Gly-Arg-pNA, and the optical density at 405 nm was measured. One unit of scu-PA was defined on the basis of 1 nmol of p-nitroaniline released from the substrate per second. One unit of scu-PA was found to be equivalent to 335 u-PA (two-chain) units (IU).

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CalorimetryDose-Response Relationship, DrugEnzyme ActivationFibrinolysinHumansOligopeptidesPlasminogen ActivatorsTime FactorsUrokinase-Type Plasminogen ActivatorJournal Article

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O Matsuo, S Ueshima, K Okada, H Fukao, M Ohgaki, R Watanabe, H Arimura. 1989. Assay method for single-chain urokinase-type plasminogen activator.. https://pubmed.ncbi.nlm.nih.gov/2531734/

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