Nucleotide sequences required for Tn3 transposition immunity.

J A Kans; M J Casadaban

doi:10.1128/jb.171.4.1904-1914.1989

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Nucleotide sequences required for Tn3 transposition immunity.

J A Kans·M J Casadaban

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Abstract

The Tn3 transposon inserts at a reduced frequency into a plasmid already containing a copy of Tn3, a phenomenon known as transposition immunity. The cis-acting site on Tn3 responsible for immunity was mapped by deletions from each side to be within the terminal 38-base-pair sequence that is inversely repeated at the ends of Tn3. Two palindromic sequences are present in the essential part of this region. Some deletions conferred only partial immunity, and others conferred negative immunity. Multiple copies of partially immune ends conferred additional immunity. No other part of Tn3 was necessary for immunity.

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Base Sequence Chromosome Mapping DNA Mutational Analysis DNA Transposable Elements DNA, Bacterial Escherichia coli Molecular Sequence Data Plasmids Recombination, Genetic Journal Article Research Support, U.S. Gov't, P.H.S.

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J A Kans, M J Casadaban. 1989. Nucleotide sequences required for Tn3 transposition immunity.. https://doi.org/10.1128/jb.171.4.1904-1914.1989

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