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Synaptosomal (Ca2+-Mg2+)-ATPASE activity modulation by cyclic AMP.

R Rodriguez·A Toledo·J Sabrià·J Rodriguez·I Blanco
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Abstract

Dibutyryl cyclic AMP, in a concentration-dependent manner, increased synaptosomal (Ca2+-Mg2+)-ATPase activity, but in synaptic plasma membranes lacked any effect. The maximal enzyme activity in synaptosomes was increased by 38%, leaving unaltered the extrasynaptosomal Ca2+ concentration necessary to reach it. In the presence of 5 microM cyclic AMP, cyclic AMP-dependent protein kinase increased (30%) maximal (Ca2+-Mg2+)-ATPase activity in synaptic plasma membranes, but the apparent affinity for Ca2+ was not modified. This effect was partially inhibited (60%) by a cyclic AMP-dependent protein kinase inhibitor. The data suggest that synaptosomal (Ca2+-Mg2+)-ATPase activity is modulated by a cyclic AMP-dependent phosphorylation reaction.

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AnimalsCa(2+) Mg(2+)-ATPaseCalciumCalcium-Transporting ATPasesCyclic AMPDose-Response Relationship, DrugFemaleProtein KinasesRatsRats, Inbred StrainsSynaptosomesJournal ArticleResearch Support, Non-U.S. Gov't

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R Rodriguez, A Toledo, J Sabrià, J Rodriguez, I Blanco. 1989-10-01. Synaptosomal (Ca2+-Mg2+)-ATPASE activity modulation by cyclic AMP.. https://doi.org/10.1016/0006-2952(89)90617-5

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