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Chronic lesions differentially decrease tyrosine hydroxylase messenger RNA in dopaminergic neurons of the substantia nigra.

G M Pasinetti·S P Lerner·S A Johnson·D G Morgan·N A Telford·C E Finch
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Abstract

Long-term effects of lesions were analyzed in terms of gene expression. Nine months after unilateral 6-hydroxydopamine (6-OHDA) lesions of the substantia nigra pars compacta (s. nigra), the remaining dopaminergic (DAergic) neurons (tyrosine hydroxylase (TH) cells determined by immunocytochemistry (ICC] on the lesioned side were atrophic with smaller nucleoli. By in situ hybridization, the DAergic neurons on the lesioned side had a 50% smaller TH-mRNA concentration than on the contralateral non-lesioned side. However, beta-tubulin mRNA concentration in DAergic neurons was unaffected by the lesion. The lesions did not alter TH-mRNA concentration in the contralateral non-lesioned side by comparison with unoperated controls. We propose that chronic lesions have long-term effects on gene expression because of damage sustained during compensatory hyperactivity after the lesion, or because of decreased trophic support from other neurons.

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AnimalsDopamineGene Expression RegulationHydroxydopaminesMaleNucleic Acid HybridizationOxidopamineRNA, MessengerRatsRats, Inbred F344Substantia NigraTyrosine 3-MonooxygenaseJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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G M Pasinetti, S P Lerner, S A Johnson, D G Morgan, N A Telford, C E Finch. 1989. Chronic lesions differentially decrease tyrosine hydroxylase messenger RNA in dopaminergic neurons of the substantia nigra.. https://doi.org/10.1016/0169-328x(89)90036-3

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