Fluorescence microscopy with antisera against specific cellular structure: double photography method for cell identification in populations of multiple cell types.

L M Cherry

doi:10.3109/10520298509113899

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Fluorescence microscopy with antisera against specific cellular structure: double photography method for cell identification in populations of multiple cell types.

L M Cherry

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Abstract

Immunofluorescent staining techniques using antitubulin antibody have been difficult to apply to meiotic tissue (testis) because of the large number of cell types present. Such techniques customarily use a fluorescent dye to counterstain nuclei, and this counterstain is hard to distinguish because of the fluorescence of the antitubulin. By counterstaining with dilute hematoxylin, we can photograph the same field using UV and then conventional illumination. This double photography allows us to identify precisely the many types of cells present, and it will be a useful tool for reexamining the staging of spermatogenesis.

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Animals Fluorescent Antibody Technique Hematoxylin Immune Sera Male Mice Mice, Inbred Strains Microscopy, Fluorescence Microtubules Photography Spermatogenesis Spermatozoa Staining and Labeling Testis Tubulin Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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L M Cherry. 1985. Fluorescence microscopy with antisera against specific cellular structure: double photography method for cell identification in populations of multiple cell types.. https://doi.org/10.3109/10520298509113899

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